LAL Reagent ama TAL Reagent ee baaritaanka endotoxin

Limulus amebocyte lysate (LAL) ama Tachypleus tridentatus lysate (TAL) waa unugyo dhiig oo biyo ah oo laga soosaaray carsaanyada fardaha.

Iyo endotoxins waa molecules hydrophobic kuwaas oo qayb ka ah iskudhafka lipopolysaccharide ee sameeya inta badan xuubka sare ee bakteeriyada Gram-negative.Alaabooyinka waalidka ee ku wasakhaysan pyrogens waxay u horseedi karaan cawaaqib xun sida qandho, shoog, xubnaha oo hawlgab, ama xataa dhimasho.

Reagent LAL/TAL waxa uu kaga falcelin karaa bakteeriyada endotoxin iyo lipopolysaccharide (LPS).LAL's endotoxins-ku-xidhka iyo awoodda xinjirowga ayaa ka dhigaysa mid aad u qiimo badan warshadaha dawooyinkayaga.Taasina waa sababta LAL/TAL reagent loo shaqaaleysiin karo si loo ogaado ama loo qiyaaso endotoxin bakteeriyada.

Kahor inta aan la helin in LAL/TAL loo isticmaali karo in lagu sameeyo baaritaanka endotoxins bakteeriyada, bakaylaha waxaa loo shaqeeyaa in lagu ogaado oo lagu qiyaaso endotoxins ee alaabta dawooyinka.Marka la barbar dhigo RPT, BET leh LAL/TAL reagent waa mid degdeg ah oo hufan, waana habka caanka ah ee lagu sameeyo la socodka firfircoon ee xoojinta endotoxin ee warshadaha dawooyinka, iyo wixii la mid ah.

Baaritaanka xinjirowga endotoxin ee gelitaanka, oo sidoo kale loo yaqaano baaritaanka Limulus Amebocyte Lysate (LAL), ama loo yaqaan Lyophilized Amebocyte Lysate (LAL) waa hab aad loo isticmaalo oo lagu ogaado laguna qiyaaso endotoxins ee alaabooyin kala duwan, gaar ahaan warshadaha dawooyinka iyo qalabka caafimaadka.Waxaa loo tixgeliyaa xal lagama maarmaanka u ah goobta ogaanshaha endotoxin sababtoo ah waxtarkeeda iyo aqbalaadda sharciyeynta.

Tijaabada LAL waxay ku salaysan tahay mabda'a ah in unugyada dhiigga ee carsaanyada fardaha (Limulus polyphemus ama Tachypleus tridentatus) ay ku jiraan cunsur xinjiro ah oo ka fal-celisa bakteeriyada endotoxins, taasoo keenta samaynta xinjir jel oo kale ah.Dareen-celintani waa mid aad xasaasi u ah oo gaar u ah endotoxins, kuwaas oo ah qaybaha sunta ah ee xuubka sare ee bakteeriyada garaam-negative.

Waxaa jira dhowr sababood oo sababta tijaabada baaritaanka xinjirowga endotoxin loo tixgeliyo xal lagama maarmaanka u ah ogaanshaha endotoxin:

1. Ogolaanshaha Nidaaminta: Imtixaanka LAL waxaa aqoonsaday oo aqbalay maamulada sharciyeynta sida USPharmacopeia (USP) iyo Pharmacopoeia Yurub (EP) oo ah habka caadiga ah ee baaritaanka endotoxin.U hoggaansanaanta xeerarkan waa qasab si loo xaqiijiyo badbaadada iyo tayada alaabta dawooyinka.

2. Dareenka iyo Gaarka: Imtixaanka LAL waxa uu leeyahay dareen sare, oo u oggolaanaya in la ogaado heerarka aadka u hooseeya ee endotoxins.Waxay awood u leedahay in ay ogaato uruurinta endotoxin sida ugu hooseysa 0.01 unugyada endotoxin halkii millilitir (EU/ml).Tijaabada gaarka ah waxay hubisaa in ay ugu horrayn ogaato endotoxins oo ay yarayso natiijooyinka beenta ah.

3. Waxtarka waxtarka leh: Baaritaanka xinjirowga gelitaanka endotoxin ee baaritaanka guud ahaan waxaa loo tixgeliyaa xal dhaqaale marka loo eego hababka kale sida baaritaanka chromogenic ama turbidimetric.Waxay u baahan tahay reagen yar iyo qalab, hoos u dhigista kharashka imtixaanka guud.Intaa waxaa dheer, helitaanka dib-u-hagaajinta LAL ee suuqa ayaa ka dhigaysa mid ku habboon shaybaarada inay sameeyaan baaritaanka.

4. Heerarka Warshadaha: Tijaabada LAL ayaa si weyn looga qaatay warshadaha dawooyinka iyo aaladaha caafimaadka sida habka caadiga ah ee ogaanshaha endotoxin.Waa qayb muhiim ah oo ka mid ah hababka xakamaynta tayada inta lagu jiro soo saarista alaabta dawooyinka iyo qalabka caafimaadka, hubinta u hoggaansanaanta shuruudaha sharciyeynta.

Si kastaba ha ahaatee, waxaa xusid mudan in tijaabada baaritaanka endotoxin ee gel xinjirowga ay yeelan karto xaddidaad, sida faragelinta walxaha qaarkood iyo suurtagalnimada natiijooyin been-abuur ah ama been-abuur ah.Xaalado gaar ah, habab kale sida chromogenic ama turbidimetric assays ayaa loo isticmaali karaa si loo dhamaystiro ama loo xaqiijiyo natiijooyinka laga helay tijaabada LAL.