Codsiga Farsamada Chromogenic ee Baaritaanka Endotoxins Bakteeriyada

Farsamada Chromogenic waxay ka mid tahay saddexda farsamo ee sidoo kale ka kooban farsamada gel-xinjirowga iyo farsamada turbidimetric si loo ogaado ama loo qiyaaso endotoxins ka bakteeriyada Gram-negative iyadoo la isticmaalayo amoebocyte lysate oo laga soo saaro dhiigga buluugga ah ee carsaanyada fardaha (Limulus polyphemus ama Tachypleus tridentatus).Waxa loo kala saari karaa sidii baadhista-dhamaadka-chromogenic ama qiimaynta kinetic-chromogenic ee ku salaysan mabda'a qiimaynta gaarka ah ee la shaqaaleysiiyay.

Mabda'a falcelinta waa in: lysate amebocyte ay ka kooban tahay qayb ka mid ah serine protease enzymes (proenzymes) kaas oo ay kicin karaan endotoxins bakteeriyada.Endotoxins-ku waxay dhaqaajiyaan proenzymes-ka si ay u soo saaraan ensaymes firfircoon (oo lagu magacaabo coagulase), kan dambe wuxuu kiciyaa kala qaybsanaanta substrate-ka aan midabka lahayn, isagoo siidaaya badeecada midabka jaalaha ah pNA.pNA-da la sii daayay waxaa lagu qiyaasi karaa sawir ahaan 405nm.Oo nuugista ayaa si togan ula xidhiidha fiirsashada endotoxin, ka dibna xoojinta endotoxin ayaa loo qiyaasi karaa si waafaqsan.