Waa maxay Baaritaanka Endotoxins?

Waa maxay Baaritaanka Endotoxins?

Endotoxins waa molecules hydrophobic kuwaas oo qayb ka ah iskudhafka lipopolysaccharide ee sameeya inta badan xuubka sare ee bakteeriyada Gram-negative.Waxa la sii daayaa marka bakteeriyadu dhimato oo xuubkeeda sare kala dillaaco.Endotoxins waxaa loo tixgeliyaa inay yihiin kuwa ugu waaweyn ee wax ku biiriya jawaabta pyrogenic.Iyo alaabooyinka parenteral ee ku wasakhaysan pyrogens waxay u horseedi karaan koritaanka qandho, kicinta jawaab-celinta bararka, shoogga, xubinta taranka iyo dhimashada aadanaha.

Baaritaanka Endotoxins waa baaritaanka lagu ogaanayo ama lagu qiyaaso endotoxins bakteeriyada Gram-negative.

Bakaylaha waxa loo shaqeeyaa in lagu ogaado oo lagu qiyaaso endotoxins ee alaabta dawooyinka marka hore.Marka loo eego USP, RPT waxay ku lug leedahay la socodka korodhka heerkulka ama qandhada ka dib duritaanka xididada ee daawada ee bakaylaha.Iyo 21 CFR 610.13(b) waxay u baahan tahay tijaabada bakayle pyrogen ee bayooloji cayiman.

Sannadihii 1960-aadkii, Fredrick Bang iyo Jack Levin waxay ogaadeen in amebocytes ee carsaanyada fardaha ay xinjiroobin doonaan joogitaanka endotoxins.TheLimulus Amebocyte Lysate(ama Tachypleus Amebocyte Lysate) ayaa loo sameeyay si waafaqsan RPT.Marka la eego USP, baaritaanka LAL waxaa loo tixraacaa baaritaanka bakteeriyada endotoxin (BET).BET-na waxaa lagu samayn karaa iyadoo la isticmaalayo 3 farsamo: 1) farsamada xinjirowga;2) farsamada turbidimetric;3) farsamada chromogenic.Shuruudaha tijaabada LAL waxay ka kooban tahay pH ugu fiican, xoogga ionic, heerkulka, iyo wakhtiga soo-baxa.

Marka la barbar dhigo RPT, BET waa mid degdeg ah oo hufan.Si kastaba ha ahaatee, BET ma beddeli karto RPT gebi ahaanba.Sababtoo ah baaritaanka LAL waxaa laga yaabaa in ay farageliyaan arrimo mana awoodo in la ogaado pyrogens aan endotoxin ahayn.