Alaabooyinka Pyrogen-ka bilaashka ah ee la isticmaalo - Tubooyinka bilaashka ah ee Endotoxin / talooyinka / microplates

Isticmaalka Pyrogen-free waa wax la isticmaalo iyada oo aan lahayn endotoxin dibadda ah, oo ay ku jiraan talooyinka pipette-free-pyrogen-free (sanduuqa caarada), tuubooyinka tijaabada ah ee bilaashka ah ama loo yaqaanno endotoxin tuubooyinka galaaska bilaashka ah, ampoules galaas-free-pyrogen-free, endotoxin-free 96-ceel microplates, iyo endotoxin- Biyaha bilaashka ah (isticmaalka biyaha depyrogenated ee baaritaanka bakteeriyada endotoxin), bakhaarka bilaashka ah ee endotoxin iyo iwm. Waxaa ka mid ah, biyaha bakteeriyada endotoxintest by habka xinjirowga gel iyo qiyaasta baaritaanka endotoxin ee dhammaan daabacadaha Pharmacopoeia ee la xiriira (USP, EP, BP, JP) iyo China Pharmaceutica).Waxa loola jeedaa biyaha nadiifka ah ee lagu muday maaddooyinka endotoxin ka yar 0.015EU/ml.Hadda nooca ugu dambeeyay ee Pharmacopoeia, biyaha BET waxay ka yar yihiin 0.005EU/ml.Xataa heerka ugu sarreeya wuxuu ka yar yahay 0.001EU/ml waxaa awood u leh inay soo saarto oo ay bixiso Bioendo.

Erayada muhiimka ah ee la xidhiidha endotoxins, iyo isticmaalka bilaashka ah ee endotoxin, pyrogen iyo ilaha kulaylku waa laba fikradood oo kala duwan:Pyrogen: Waxa kale oo loo yaqaan 'pyrogen' ama 'exothermic factor'.walxaha keeni kara korodhka heerkulka jidhka.Isha kulaylka: shay kuleyl siiya.Sida taraqa gubanaya, dhuxusha iwm.Waxa loogu yeero "consumables non-pyrogenic" iyo "jawaabta pyrogenic" ee qaar ka mid ah soosaarayaasha iyo baayacmushtarka ayaa dhab ahaantii ah magacyo aan xirfad lahayn oo marin habaabin ah.Kuwa saxda ah waa inay ahaadaan "Pyrogen Free" iyo "Jawaabta Pyrogen".

Waa maxay sababta ay alaabada bilaashka ah ee Pyrogen-ka ay lagama maarmaanka u tahay baaritaanka endotoxin ee baaritaanka, labadaba gelitaanka xinjirowga endotoxin tijaabada baaritaanka iyo baaritaanka tirada endotoxin?

Haa, maaddooyinka aan pyrogen-ka lahayn ayaa lagama maarmaan u ah samaynta baaritaanka endotoxin ee si sax ah oo la isku halleyn karo.Jiritaanka pyrogens, kuwaas oo ah walxaha qandhada keena inta badan laga helo bakteeriyada endotoxins, waxay faragelin kartaa natiijada baaritaanka waxayna horseedi kartaa akhrin been ah.Tijaabada endotoxin, oo loo yaqaanno Limulus amebocyte lysate (LAL) ama loo yaqaan baaritaanka Lyophilized amebocyte lysate (LAL), ayaa loo isticmaalaa in lagu ogaado oo lagu qiyaaso joogitaanka endotoxins bakteeriyada ee dawooyinka, aaladaha caafimaadka, iyo alaabada kale.Tijaabada LAL waxay ku tiirsan tahay falcelinta u dhaxaysa reagent LAL iyo endotoxins si ay u soo saarto jawaab celin xinjiro ama koromojiin ah.Si loo xaqiijiyo natiijooyinka saxda ah, waxaa muhiim ah in la isticmaalo waxyaabaha la isticmaalo oo ka madax bannaan pyrogens.Pyrogens waxa ay wasakhayn kartaa agabka shaybaadhka ee kala duwan, oo ay ku jiraandhalooyinka, talooyinka pipette, tubooyinka, iyo weelasha muunada.Haddii maaddooyinka wasakhaysan ee pyrogen-ku ay la xiriiraan reagent LAL ama shaybaarrada tijaabada ah, waxay kicin karaan falcelinno been abuur ah, taasoo horseedaysa gabagabo khaldan oo ku saabsan joogitaanka ama uruurinta endotoxins.Alaabooyinka Pyrogen-la'aanta ah ayaa la soo saaray oo si gaar ah loo tijaabiyaa si loo yareeyo ama loo baabi'iyo joogitaanka pyrogens.Waxay maraan hababka xakamaynta tayada adag si loo hubiyo inay buuxiyaan heerarka looga baahan yahay baaritaanka endotoxin.Isticmaalka alaabooyinkan gaarka ah waxay gacan ka geysaneysaa ilaalinta sharafta iyo saxnaanta baaritaanka endotoxin, hubinta natiijooyinka la isku halleyn karo iyo kor u qaadista badbaadada bukaanka ee codsiyada dawooyinka iyo biomedical.