Baaritaanka Endotoxin Test Assay by Lyophilized Amebocyte Lysate (LAL Reagent)

Baaritaanka Endotoxin Test Assay by Lyophilized Amebocyte Lysate (LAL Reagent)

LAL ReagentLyophilized amebocyte lysate (LAL) waa dheecaan laga soosaaray unugyo dhiiga (amebocytes) oo laga soosaaray carsaanyada caleenta Atlantiga.
TAL Reagents: TAL reagent waa dheecaan laga soosaaray unugyada dhiiga ee Tachypleus tridentatus.
Waqtigan xaadirka ah, wax soo saarka ugu weyn ee LAL/TAL reagents waa Maraykanka iyo Shiinaha.

Gel clot endotoxin test assay, habkani weli waa codsiyada ugu muhiimsan ee adduunka oo dhan, si loo buuxiyo shuruudaha badbaadada ee daawooyinka cirbadeynta Aadanaha.
HADDA Bioendo waxay soo saartaa oo siisaa jel xinjireedka LAL reagent oo ay ku jiraan dhalooyinka quraarad ee tijaabada ah iyo dhalooyin badan oo tijaabo ah.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 iyo G52
Tani waa xalka dhaqaale ee lagu ogaanayo endotoxins tayada leh ee tijaabada daroogada.Gaar ahaan dawooyinka la isku duro ama daawooyinka parenteral si loogu tijaabiyo endotoxins gudaha WFI, API ama alaabada dawooyinka dhammaatay.Baaritaanka Endotoxin test assay waa baahida sare ee ficilada hawlgalka, waxay u baahan tahay hawl wadeen aqoon u leh si uu ula tacaalo baaritaanka xinjirowga jel si loo xaqiijiyo dib u soo celinta saxda ah.

Xalka dhamaystiran eeG52Baaritaanka Endotoxin:
LAL Reagent
Xakamaynta Heerka Endotoxin
BET Biyaha
Talooyinka pipette-free endotoxin
Tuubooyinka dhalooyinka ee aan lahayn Endotoxin, oo ay ku jiraan qalliinka milanka iyo tuubooyinka falcelinta.
Qalabka wax-soo-saarka, si loogu taliyo qubeyska biyaha ama kuleelka qalalan.Dhammaan aaladaha soo-saarka waxay u baahan yihiin saxnaanta heerkulka.
Dhammaan alaabta la isticmaalo si loo taabto baaritaanka LAL kaas oo buuxiya heerka heerka endotoxin-free " <0.005EU/ml" .
Deegaanka tijaabada ahi waa inuu ku habboonaado ogaanshaha endotoxin.

U fiirso:
Alaabta caagga ah sida talooyinka pipette ama taarikada multiwell ayaa si weyn loogu isticmaalaa baaritaanka endotoxin.Pharmacopoeias-is-waafajinta (USP/CP) waxay u baahan tahay in wax kasta oo caag ah oo la isticmaalo iyo tuubooyinka dhalada ah ay tahay inay xor ka ahaadaan endotoxin la ogaan karo oo si la mid ah muhiim laakiin inta badan aan la tixgelin, waa inay ahaadaan heerka bilaashka ah ee arrimaha faragelinta.

Sida loo isticmaalo baaritaanka endotoxin ka si loo ogaado endotoxins muunada?
Ugu horreyntii, baaritaanka xaqiijinta dareenka lysate ee calaamadeysan waa in la sameeyaa.Si loo xaqiijiyo dareenka waxay la mid tahay calaamadaha summada.
Falanqaynta muunada, samee baaritaanada baaritaanka endotoxin kahor faragelinta ka hor.
Si loo sameeyo baaritaanka endotoxin oo dhammaystiran ee "tijaabada faragelinta".
Si loo sameeyo tijaabada xadka si loo helo sida uu yahay heerka endotoxins ee muunada.

Marka la hawlgaliyo tijaabada xaqiijinta dareenka lysate ee calaamadeysan, natiijadu waa mid aan caadi ahayn, 2 Lamada ma helin samaynta jel?
Hubi in habka diyaarinta ee heerka xakamaynta bakteeriyada endotoxin uu sax yahay.
Isku darka Vortex ayaa looga baahan yahay dareere kasta (eeg buug-tilmaameedka ama loo yaqaan galinta heerka endotoxin ee faahfaahinta).
Si loo hubiyo in reagent-ka lysate uu ku habboon yahay heerka xakamaynta endotoxin.

Waxaa lagu talinayaa in la isticmaalo qubayska biyaha ama kulaylka qallalan ee habka heerkulka joogtada ah ee reagent lysate (tusaale ahaan, incubator ama sanduuq qalalan ma aha mid waxtar leh).
Inta lagu jiro soo noqnoqoshada, deegaanka biyaha ee qubeyska biyaha oo ah mid taagan oo deggan, dhammaan gariirku waa la iska ilaaliyaa.
Si gaar ah u fiirso biyaha qubeyska si aad u damiso bamka socodka biyaha.

Marka la qiimeeyo natiijada, si tartiib ah uga soo saar tuubada tijaabada ama ampoule dhalada ah heerkulbeegga, si tartiib ah u rogo 180 darajo,
samaynta jel ee tuubada ma noqon doonto mid qallafsan, mana simbiriirixi doonto, natiijada waxaa lagu duubay calaamad "+";
ma samaysma jel ama xinjirta jel lama sii hayn karo inkasta oo ay samaysan tahay.
Dharka darbigu waa taban, natiijada waxaa lagu duubay calaamad "-".
Xinjiraha xinjirowga ah ee degdega ah Qalabka tijaabada xinjirowga ee endotoxin waxaa iska leh habka baaritaanka xinjirowga jel.
Baaritaanka xinjirowga jel ee degdega ah ee endotoxin, muunadda xakamaynta togan ma dhaliso jel?
Marka hore, isticmaal biyaha tijaabada bakteeriyada endotoxin si aad u hubiso reagents lysate, hubi in hawlwadeenada iyo deegaanku ay buuxiyeen shuruucda;

Haddii qalabku u qalmo, markaa xakamaynta togan ee shaybaarku ma samaynayso gel sababtoo ah saamaynta xannibaadda ee muunadda, muunadda waa in la farsameeyaa oo la daaweeyaa.
Habka muunada ee ugu caansan waa qasid