Talooyinka pipette bilaashka ah ee Pyrogen – Tubooyinka tijaabada bilaashka ah ee Endotoxin / Talooyin / microplates

Isticmaalka Pyrogen-la'aanta ah waa wax la isticmaalo iyada oo aan lahayn endotoxin dibadda ah, oo ay ku jiraan talooyinka pipette-free-pyrogen-free (sanduuqa caarada), tuubooyinka tijaabada ah ee bilaashka ah ama loo yaqaanno endotoxin tuubooyinka galaaska bilaashka ah, ampoules galaas-free-pyrogen-free, endotoxin-free 96-ceel microplates, iyo endotoxin- biyo bilaash ah (isticmaalka biyaha depyrogenated ee baaritaanka bakteeriyada endotoxin), bakhaar bilaash ah endotoxin iyo iwm. Waxaa ka mid ah, biyaha loogu talagalay endotoxintest bakteeriyada by habka xinjirowga gel iyo qiyaasta baaritaanka endotoxin ee dhammaan daabacadaha Pharmacopoeia ee la xiriira (USP, EP, BP, JP iyo China Pharmaceutica) . Waxa loola jeedaa biyaha nadiifka ah ee lagu muday maaddooyinka endotoxin ka yar 0.015EU/ml. Hadda nooca ugu dambeeyay ee Pharmacopoeia, biyaha BET waxay ka yar yihiin 0.005EU/ml. Xataa heerka ugu sarreeya wuxuu ka yar yahay 0.001EU/ml waxaa awood u leh inay soo saarto oo ay bixiso Bioendo.

Sharaxaaddu waxay bixinaysaa dulmar tifaftiran oo ku saabsan isticmaalka aan lahayn pyrogen-ka iyo codsiyadooda, gaar ahaan xoojinta doorkooda muhiimka ah ee baaritaanka endotoxin ee heerarka kala duwan ee pharmacopoeia. Halkan waxaa ah qodobbada muhiimka ah oo kooban:

1. Qeexid:
   • Cunnooyinka aan lahayn Pyrogen-ka waa shay aan lahayn endotoxins dibadda ah, oo ay ku jiraan:
     • Talooyinka pipette-free-yrogen-free(sanduuqa caarada).
     • Tuubooyinka tijaabada ah ee bilaashka ah ee Endotoxin ama tuubooyinka dhalooyinka.
     • ampoules galaas aan lahayn Endotoxin.
     • Endotoxin-free 96-ceel microplates.
     • Biyo aan lahayn endotoxin (biyaha depyrogenated).
     • Xirmooyinka aan lahayn endotoxin
2. Biyaha loogu talagalay Baaritaanka Endotoxin Bakteeriyada (BET):
   • Loo isticmaalo hababka xinjirowga jel iyo tijaabooyinka endotoxin ee tirada sida ku cad USP, EP, BP, JP, iyo China Pharmacopeia heerarka.
   • Heerka hore: ≤0.015 EU/ml ee biyaha nadiifka ah ee duritaanka.
   • Shuruudaha hadda (pharmacopoeia ee ugu dambeeyay): ≤0.005 EU/ml ee biyaha BET.
   • Heerka sare: Bioendo wuxuu soo saari karaa biyo leh ≤0.001 EU/ml content endotoxin, buuxinta shuruudaha tayada ugu sarreeya.

Soo jeedinta dib-u-habaynta:

• Xooga saar baaxadda codsiga, tusaale, shaybaadhada, daawooyinka, ama bayotechnoolajiyada.
• Bixi faahfaahin dheeraad ah oo ku saabsan khibradda Bioendo ee wax soo saarka si loo xoojiyo geeskeeda tartanka.
• Muuji faa'iidooyinka gaarka ah ee alaabta Bioendo ee heerka sare ah, sida saxnaanta qiimaynta oo la xoojiyay ama u hoggaansanaanta sharciga.

Ma jeclaan lahayd in aan kaa caawiyo samaynta tan si aan uga dhigo badeecad rasmi ah ama sharraxaad farsamo?

Erayada muhiimka ah ee la xidhiidha endotoxins, iyo isticmaalka bilaashka ah ee endotoxin, pyrogen iyo ilaha kulaylku waa laba fikradood oo kala duwan:Pyrogen: Waxa kale oo loo yaqaan 'pyrogen' ama 'exothermic factor'. walxaha keeni kara korodhka heerkulka jidhka.Isha kulaylka: shay kuleyl siiya. Sida taraqa gubanaya, dhuxusha, iwm. Waxa loogu yeero "consumables non-pyrogenic" iyo "jawaab celinta pyrogenic" ee qaar ka mid ah soosaarayaasha iyo baayacmushtarka ayaa runtii ah magacyo aan xirfad lahayn oo marin habaabin ah. Kuwa saxda ah waa inay ahaadaan "Pyrogen Free" iyo "Jawaabta Pyrogen".

Waa maxay sababta ay alaabada bilaashka ah ee Pyrogen-ka ay lagama maarmaan u tahay baaritaanka endotoxin ee baaritaanka, labadaba gelitaanka xinjirowga endotoxin test assay iyo tijaabada baaritaanka endotoxin tirada labadaba?

Haa, maaddooyinka aan lahayn pyrogen-ka ayaa lagama maarmaan u ah samaynta baaritaanka endotoxin si sax ah oo la isku halleyn karo. Joogitaanka pyrogens, kuwaas oo ah walxaha qandhada keena inta badan laga helo bakteeriyada endotoxins, waxay faragelin kartaa natiijada baaritaanka waxayna horseedi kartaa akhrin been ah. Baaritaanka endotoxin, oo loo yaqaanno Limulus amebocyte lysate (LAL) ama loo yaqaan Lyophilized amebocyte lysate (LAL), waxaa loo isticmaalaa in lagu ogaado oo lagu qiyaaso joogitaanka endotoxins bakteeriyada ee dawooyinka, qalabka caafimaadka, iyo alaabada kale. Tijaabada LAL waxay ku tiirsan tahay falcelinta u dhaxaysa reagent LAL iyo endotoxins si ay u soo saarto jawaab celin xinjir ama chromogenic. Pyrogens waxa ay wasakhayn kartaa agabka shaybaadhka ee kala duwan, oo ay ku jiraandhalooyinka, talooyinka pipette, tubooyinka, iyo weelasha muunada. Haddii alaabta wasakhaysan ee pyrogen-ku ay la kulmaan reagent LAL ama shaybaarada tijaabada ah, waxay kicin karaan falcelin been abuur ah, taasoo horseedaysa gabagabo khaldan oo ku saabsan joogitaanka ama xoojinta endotoxins joogitaanka pyrogens. Waxay maraan hababka xakamaynta tayada adag si loo hubiyo inay buuxiyaan heerarka looga baahan yahay baaritaanka endotoxin. Isticmaalka alaabtan gaarka ah waxay gacan ka geysataa ilaalinta daacadnimada iyo saxnaanta baaritaanka endotoxin, hubinta natiijooyin la isku halayn karo iyo kor u qaadista badbaadada bukaanka ee codsiyada dawooyinka iyo biomedical.