Hagaha iibka ee Bioendo End-point Chromogenic LAL Test Assay Kit

HanuunintaQalabka Qiimaynta Baadhista Chromogenic LAL ee Dhibcaha Dhammaadka Bioendo:

TAL reagent, ie lyophilized amebocyte lysate oo laga soosaaray dhiiga buluuga ah ee carsaanyada fardaha (Limulus polyphemus ama Tachypleus tridentatus), ayaa had iyo jeer loo shaqeeyaa in lagu sameeyo baaritaanka endotoxins bakteeriyada.

Markay tahay Bioendo, waxaanu soo saarnay qalabyo si aanu u fulino qiimaynta-chromogenic TAL assay/LAL-ka, oo ay ku jiraan dhammaan reagen-yaasha lagama maarmaanka u ah BET iyada oo loo marayo isbeddelka horumarinta midabka, oo u oggolow macaamilka inuu helo natiijooyin sax ah muddo gaaban gudaheed.

Mabaadi'da ogaanshaha chromogenic-ka barta-dhamaadka leh ee TAL reagent waa kuwa: falcelinta enzymatic waxay dhacdaa marka bakteeriyada endotoxins ay kiciso factor C, iyo xinjirowga ayaa loo sameeyay si waafaqsan.Kadib xinjirowga wuxuu u kala qaybiyaa substrate aan midab lahayn si uu u sii daayo badeecada midabka jaalaha ah pNA.pNA-da la sii daayay waxaa lagu qiyaasi karaa sawir ahaan 405nm.Maadaama nuugista ay si togan ula xiriirto fiirsashada endotoxin, fiirsashada endotoxin ayaa lagu qiyaasi karaa si waafaqsan.

EC64405, ie BioendoEC Qalabka Tijaabada Endotoxin (Dhibca Dhamaadka Chromogenic Assay), waa xidhmada qiimaynta Chromogenic TAL ee dhamaadka-dhibcaha oo lagu dabaqi karo in lagu ogaado qiyaasta endotoxin bakteeriyada ku jirta muunada.Xirmada EC64405 ee ay soo saartay Bioendo waxay ogolaataa 64 imtixaan, dareenka dareenkuna wuxuu ka bilaabmaa 0.1 EU/ml ilaa 1 EU/ml.Bioendo EC64405 waxay ka kooban tahay dhammaan reagen-yaasha looga baahan yahay qiimaynta chromogenic-ka.Maadaama aan sidoo kale haysanno qalabyada endotoxin-free ama pyrogen-free, waxaan sidoo kale xirxi karnaa xirmada iyadoo ku saleysan waxaad u baahan tahay.

Soo-saarista Microplate Reader ayaa looga baahan yahay qiimeynta chromogenic-ka ee dhibicda-dhamaadka.Akhristahayada microplate-ka soo-saarka ELx808IULALXH (qaab cusub oo la shaqeynayo oo dhawaan la bilaabi doono) wuxuu u oggolaanayaa muunado kala duwan in lagu ogaado 96-si fiican microplate isku mar waxayna u baari doontaa ogaanshaha endotoxin si toos ah oo sax ah.

Baaritaanka LAL-ka-dhamaadka-chromogenic-ka ee tijaabada ah oo leh laba taxane oo xirmo ah, mid wuxuu adeegsadaa akhristaha microplate si uu u akhriyo natiijada qiyaasidda microplate-free-pyrogen-ka, kan kalena wuxuu isticmaalaa spectrophotometer-ka caadiga ah si uu u akhriyo natiijada tuubooyinka dhalooyinka aan endotoxin-free.

Lyophilized Amebocyte Lysate / LAL Reagent oo ay soo saartay Bioendo waxa laga sameeyay amebocyte lysate oo ka soo jeeda dhiigga carsaanyada fardaha.