Qalabka ka saarida EtEraser™ HP Endotoxin

Qalabka ka saarida EtEraser™ SE Endotoxin

1. Macluumaadka alaabta

Waxaan nahay khabiirka baaritaanka endotoxin iyo ka saarida endotoxin.Waxaan bixinaa xalal dhamaystiran oo daboolaya dhammaan noocyada kala duwan ee xakamaynta endotoxin, la socodka endotoxin iyo tijaabinta.Alaabadayada waxaa ka mid ah isticmaalka cilmi-baarista shaybaarkaxirmada ka saarista endotoxins waxaana lagu qiyaasi karaa ilaa tiirarka miisaanka wax soo saarka warshadaha.Lipopolysaccharide (LPS) waa bakteeriyada endotoxin waana qayb weyn oo ka mid ah derbiyada unugyada bakteeriyada garaam-negative.Recombinantprotein ka E.coliu caadi ahaan waxa ku jira endotoxins heer sare ah.Ka-soo-baxa endotoxins-yadan ayaa aad lagama maarmaan u ah hababka hoose.

Qalabka saarista EtEraser HP Endotoxinwaxaa loogu talagalay in laga saaro wasakheynta endotoxin ee xalalka aqueous.Xirmooyinka waxaa ku jira resinbinds saarista endotoxin si loo yareeyo heerarka endotoxin ee shaybaarada borotiinka ≥99% in ka yar 2saacadood.

Xirmadan ka saarista endotoxin waxaa loo isticmaali karaa borotiinka, DNA/RNA, polysaccharide iyo muunado kale oo bayooloji ah.

2. Tilmaamaha badeecada

◆ Deganaanshuhu sare — ma saameeyo dhaqdhaqaaqa inta badan muunadda noolaha

◆ Soo kabashada borotiinka sare -> 95% soo kabashada borotiinka ee muunada borotiinka

◆ Hufnaanta sare ee saarista - saar> 99% endotoxin, heerka endotoxin ee muunadku wuxuu noqon karaa wax ka yar 0.1 EU/mafter habka saarista endotoxin

◆ kala duwanaansho ballaaran - waxaa loo isticmaali karaa ka saarida endotoxin ee borotiinnada, peptides, unugyada difaaca jirka, tallaalada, polysaccharide iyo shaybaarada kale ee noolaha.

EtEraserHP Xirmada ka saarista endotoxin-sare ee waxtarka leh waxay isticmaashaa isku xidhka xidhidhiyaha aan dhaqaaqin ee PMB si loo xidho ugana saaro endotoxin ka soo baxa aqueous.Isku-xidhka PMB (polymyxin B) ee la beddelay waa ligand endotoxin gaar ah oo gaar ah.Tiirka wuxuu leeyahay heerka soo kabashada borotiinka oo aad u sarreeya in ka badan 95%.Heerka Endotoxin ee shaybaarku wuxuu noqon karaa wax ka yar 0.1 EU/ml ka dib habka ka saarista endotoxin.

Kitinku waxa ku jira tiir ka saarista endotoxin oo hore loo sii diyaariyey oo ah 1.5 ml, Qalabaynta Qalabaynta, Dib-u-Celinta Dib-u-Celinta iyo tuubada ururinta aan lahayn pyrogen iyo talooyin.Tiirku waxa uu leeyahay awood xidhid sare>2,000,000 EU/ml.Alaabtan waxa laga yaabaa in dib loo isticmaalo ilaa shan jeer haddii si habboon loo soo cusboonaysiiyo.Xabagta affinity-ga ayaa diyaar ah waxaana laga yaabaa inay kor u kacdo habka biopharmaceutical.